It’s one of four matches to take place this week and to be shown live on BT Sport with highlights on ITV.
The Gunners hope it will be a case of third time lucky as the Premier League side have lost their two previous two-legged knockout ties against Barcelona, who also won in England at this stage of the competition in 2014 and 2015.
Arsenal did came from behind to beat Barcelona 2-1 when the teams last met in London in the 2010/11 round of 16, with Robin van Persie and Andrey Arshavin overturning David Villa’s first-half goal.
Elsewhere, Dynamo Kiev take on Manchester City at the Olympic Stadium, PSV Eindhoven face Atletico Madrid at the Philips Stadion, while Bayern Munich travelled to face Juventus in Turin.
Arsenal v Barcelona is live on BT Sport Europe from 7.00pm with live streaming on BTSport.com and via the BT Sport App. Kick-off 7.45pm.
Arsenal v Barcelona
Tues 23 Feb – BT Sport Europe, 7.00pm – ko 7.45pm
Juventus v Bayern Munich
Tues 23 Feb – BT Sport 2, 7.00pm – ko 7.45pm
Dynamo Kiev v Manchester City
Wed 24 Feb – BT Sport Europe, 7.00pm – ko 7.45pm
PSV Eindhoven v Atletico Madrid
Wed 24 Feb – BT Sport 2, 7.00pm – ko 7.45pm
UEFA Champions League Highlights
Wed 24 Feb – ITV, 11.05pm
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